DNA assay based on Nanoceria as Fluorescence Quenchers (NanoCeracQ DNA assay)
نویسندگان
چکیده
منابع مشابه
Fluorescence-based, high-throughput DNA polymerase assay.
The commonly used DNA polymerase assay is based on the detection of incorporated radiolabeled nucleotides in a DNA elongation reaction. It is laborious, radioactive, and can be highly variable. Here we report a nonradioactive fluorescence-based assay. The method consists of Cydye-labeled nucleotides, biotinylated primer, and a streptavidin-coated microplate. The assay is found to have sensitivi...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-20659-9